OPTIMIZATION OF ITRAQ LABELLING COUPLED TO OFFGEL FRACTIONATION AS A PROTEOMIC WORKFLOW TO THE ANALYSIS OF MICROSOMAL PROTEINS OF MEDICAGO TRUNCATULA ROOTS

Abstract Background Shotgun proteomics represents an attractive technical framework for the study of membrane proteins that are generally difficult to resolve using two-dimensional gel electrophoresis.The use of iTRAQ, a set of amine-specific isobaric tags, is currently the labelling method of choice allowing multiplexing of up to eight samples and

The c-myc proto-oncogene is induced by mitogens and is a central regulator of cell growth and differentiation.The c-myc product, Myc, is a transcription factor that binds a multitude of genomic sites, estimated to be over 10-15% of all promoter regions.Target promoters generally pre-exist in an red pygmy dogwood active or poised chromatin state tha